Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses
نویسندگان
چکیده
منابع مشابه
Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages.
The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by us...
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The Parechovirus genus of the Picornaviridae family contains two species, Human parechovirus (HPeV) and Ljungan virus (LV). The HPeVs (including the former echoviruses 22 and 23, now HPeV type 1 (HPeV1) and HPeV2, respectively) cause a wide spectrum of disease, including aseptic meningitis, gastroenteritis, encephalitis, acute respiratory illness, and neonatal sepsis-like disease. The LVs were ...
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متن کاملApplication of Real-time Polymerase Chain Reaction (RT-PCR)
The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2018
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2017.10.010